Abstract
Tissue adhesives have been prepared from fibrinogen, one of the clotting elements of blood. This material is commercially available as a closing, bonegraft fixation, skin graft fixation, and other soft tissue fixation. On the other hand, the adhering strength of the fibrin glue has not been necessarily achieved until now, though many studies have been trying to improve the adhering strength. In this study, we tried to improve the adhering strength of fibrin glue by blending appropriate amount of soluble polypeptides, atero-collagen as a natural polypeptide as well aspoly (L-glutamic acid) as a synthetic polypeptide, into the fibrinogen aqueous solution.In addition, we studied whether it is effective to apply ultrasonic vibration technique just after mixing adhesives for a short time improving the adhering strength. It was found that the adhering strength of fibrin glue containing soluble polypeptide increased almost 2 times compared to the case without the polypeptide. Application of ultrasonic vibration onto the adhesive site for1 min has been also found to be effective for increasing the adhering strength of the fibrin glue.
