Applied Entomology and Zoology
Online ISSN : 1347-605X
Print ISSN : 0003-6862
ISSN-L : 0003-6862
Regular Papers
Discrimination of three cyst-forming nematodes of the genus Globodera (Nematode: Heteroderidae) from Japan based on PCR-RFLP of ribosomal DNA
Taketo UeharaAtsuhiko KushidaKenji ItouTakashi NarabuYoji Momota
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2005 Volume 40 Issue 3 Pages 537-543

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Abstract

The ITS1-5.8S-ITS2 region of rDNA sequences from three Globodera species found in Japan, G. rostochiensis, G. tabacum and G. hypolysi, were sequenced and compared. Sequence lengths for G. rostochiensis, G. tabacum and G. hypolysi were 1,001, 1,003 and 1,016 bp, respectively. Sequence divergence within the three Globodera species ranged from 3.8 to 14.7%. Eight restriction enzymes (AfaI, AluI, FspI, HhaI, HinfI, MvaI, PmaCI and SacII) were selected on the basis of sequence information. These three species were easily distinguished according to species-specific patterns by digesting the amplified products with AluI or PmaCI. The FspI-digested pattern distinguished G. rostochiensis from the other Globodera species.

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© 2005 by the Japanese Society of Applied Entomology and Zoology
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