2008 Volume 43 Issue 4 Pages 593-598
We investigated whitefly species and their biotypes, PCR-positive individuals of Tomato yellow leaf curl virus (TYLCV), and leaf curl symptoms by collecting whitefly adults and observing the leaves of various crops in winter greenhouses in Shizuoka Pref., Japan. From January to February 2006, 48 collection sites and 5 kinds of crops were investigated. Leaf curl symptom was observed in 23 out of 36 tomato greenhouses. The whitefly in tomato greenhouses were collected from 23 Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) B biotype populations and 12 Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) populations. The B. tabaci Q biotype was detected in only 2 separate poinsettia greenhouses. From strawberry and melon greenhouses, only Trialeurodes sp. and B. tabaci B biotype, respectively, were collected. TYLCV was detected in 13 out of 30 B. tabaci B biotype populations and in 6 out of 6 T. vaporariorum populations collected from tomato, poinsettia, and melon greenhouses, irrespective of the presence of leaf curl symptom. TYLCV was not detected in any B. tabaci Q biotype individuals.