Abstract
The Henosepilachna vigintioctomaculata complex is complicated. Besides H. vigintioctomaculata (=Hv) and H. pustulosa(=Hp), other questionable forms, the Western Tokyo form (=Ht) and Towada form epilachnas(=Hc), are found in Japan. Variation in the blood acid phosphatase and esterase isozymes of this group was examined using the disc electrophoresis technique on acrylamide gel. Judging from the number of isozyme bands per individual in each population, the Hv-complex was in the sequence of Hv<Hp<Ht≒Hc. The phylogenetical arrangement of this group based on acid phosphatase isozyme was shown. The Hv-complex was divided into two main groups, one being Hv and the other Hp, Ht and Hc. Of the latter three, Ht and Hc were found to bear a close relationship.
