A Method for the Detemination of Respiratory Quotient by Gas Chromatography
1984 Volume 19 Issue 4 Pages 456-460
Details
1984 Volume 19 Issue 4 Pages 456-460
A gas chromatographic method for the determination of respiratory quotient was described. The system employed two columns in series, the filled with Porapak Q[○!R] separated CO2 from air, and the second packed with molecular sieve 5A separated O2 from N2. Each gas component was measured with a thermal conductivity detector; slight differences between the concentrations were detected and the reproducibility was good. Effects of the O2 concentration on the respiration of a wolf spider, Pardosa astrigera (L. KOCH), were investigated. Reduction in the O2 concentration to 17-18% signifcantly depressed the respiration. The results suggest that the O2 concentration inside the respiratory chambers should not decrease below this critical value.
