Abstract
The calling and mating behavior of the lesser mulberry pyralid, Glyphodes pyloalis WALKER were observed. The mean mating time preceded the mean calling time by 3.5 to 5 hr on day 2 to day 4 after emergence. The males did not show any stereotyped mating dance when the females began calling. The attraction of males to the females was therefore used as a criterion for the sex pheromone bioassay. In the laboratory bioassay, the males strongly responded and were attracted to virgin females or the abdominal tip extract of virgin females. In the field-cage bioassay also, ca. 88% of the released males were attracted and trapped in the virgin female trap, and ca. 40% in the crude extract trap.