Abstract
In Coccinella septempunctata bruckii found specifically in Japan, diapause regulation was studied by the electrophoretic pattern of haemolymph protein. Specific protein bands were found respectively with induction of aestivation and hibernation. With termination of dormancy, these specific bands disappeared and a band corresponding to vitellogenin (Vg) appeared in the females. The bands specific to diapause were also found in females reared at 25°C under long photoperiod after hatching. Topical application of juvenile hormone analogue to aestivating females revealed the Vg band and caused disappearance of the diapause specific bands. Observation indicates that a summer condition affect the corpus allatum activity of adults and causes a change in the electrophoretic pattern of haemolymph protein characteristic of diapause.
