Abstract
A simple method was considered to estimate population size from a release-recapture experiment where marked individuals are released only once and all of the captured individuals are removed from the population. This method was applied to a population estimation of Spodoptera litura (FABRICIUS) (Lepidoptera: Noctuidae). The estimates were compared with those obtained by the JOLLY-SEBER method which requires a multiple-release experiment. The results of our conservative estimation were similar to those obtained by the JOLLY-SEBER method, which suggested the practical usefulness of our method.