Determining Gene Location on Chromosomes of Bombyx mori Using Fluorescence in situ Hybridization (FISH)

Abstract

Fluorescence in situ hybridization (FISH) has been used as the technique for gene localization on chromosomes of Bombyx mori. This is particularly useful due to the difficulty in constructing chromosome maps in Bombyx mori. We could detect r DNA as one large cluster on pachytene and metaphase chromosomes at the first meiosis and the interphase nucleus of the spermatocyte using fluorescein isothiocyanate (FITC). A single copy gene, egg specific protein (ESP) gene, was detected using 7-amino-4-methylcoumarin-3-acetic acid (AMCA).