Abstract
In a 3-min methanol wash of field-collected millipede, Nedyopus patrioticus patrioticus (ATTEMS, 1898), the following four components were identified by GLC, HPLC, GC/MS and NMR as possible defense substances: benzaldehyde, phenol, p-cresol and mandelonitrile, among which p-cresol was the major component in adults (282 μg on average in males and 300μg in females), and was found in trace amounts (0.2 μg on average) in the nymphal stages. This is the first case among Polydesmida millipedes whereby a large amount of -cresol was contained as the adult specific compound and phenol as the female biased one. Hydrogen cyanide was also detected from this species.