Abstract
Reconstruction of 3D image by using a plural photograph with the different focal length is often requested in the field of agricultural information research. As an example, an image processing system for 3D image display of cytoplasmic strands orientation in a cell was constructed.
In the present paper, the system used 4 microscope photographs of a single barley coleoptile cell taken at 10 μm intervals in depth and recognized automatically the indentical strands found in different photographs as a single strand in 3D, and finally displayed a 3D image of strands in a cell. By comparing the inforamation of strands with the results of cytoplasmic streaming obtained before, it was shown that cytoplasmic strands were oriented radially when cytoplasmic streaming was most active.