Abstract
Stimulation of the focused gene by a medicine or a functional protein and to detect mRNA expression levels were very useful not only for the biologically but also for the progress of medical therapy. Furthermore, mRNA transcription suppression by antisense also clearized and made possible the speedy result of the cell biological investigation. We created primer design protocol using fuzzy deducing theory, because the most of the invented computer protocol succeeded less than 20% that was orally been tradition. The 90% of our primers functioned after checking (1. GC Contents Check; 2. Homologous Check: [versus a.: GAPDH, b: Pro-filin, c: Beta-actin]; 3. Self-Homology Check;). We also added 4: 5'-, & -3' homology checking, and 5: gene family checking to target into a real mRNA out of similar sequences. Fuzzy deducing checking protocol made possible to select real VEGF mRNA from gene family in which had to show many similar sequences during alcoholic rat liver fibrogenesis.
