MECHANISM OF PROTEIN SYNTHESIS INHIBITION BY BOTTROMYCIN A₂ : STUDIES WITH PUROMYCIN

Abstract

Bottromycin A₂ inhibits polylysine synthesis with poly A at low concentrations of E. coli ribosomes. Peptide release by puromycin from ribosomes was significantly inhibited by blasticidin S, chloramphenicol and thiophenicol, but not by bottromycin A₂ in the absence of GTP and G factor. Puromycindependent release of peptide from ribosomes was stimulated by the presence of GTPand G factor; it was inhibited by bottromycin A₂. The results indicate that bottromycin A₂ inhibits translocation of peptidyl-tRNA on the ribosomes, but does not affect peptide bond synthesis.