THE SPECTROPHOTOMETRIC DETERMINATION OF 5-METHYL-3-(2, 6-HALOGEN SUBSTITUTED PHENYL)-4-ISOXAZOLYL PENICILLINS (CLOXACILLN, DICLOXACILLIN AND FLUCLOXACILLIN)

TSUTOMU YASUDA; SHIGEYUKI SHIMADA

doi:10.7164/antibiotics.24.290

The Journal of Antibiotics

Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820

THE SPECTROPHOTOMETRIC DETERMINATION OF 5-METHYL-3-(2, 6-HALOGEN SUBSTITUTED PHENYL)-4-ISOXAZOLYL PENICILLINS (CLOXACILLN, DICLOXACILLIN AND FLUCLOXACILLIN)

TSUTOMU YASUDA, SHIGEYUKI SHIMADA

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1971 Volume 24 Issue 5 Pages 290-293

DOI https://doi.org/10.7164/antibiotics.24.290

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Abstract

A method is described for the determination of 5-methyl-3-(2, 6-halogen substituted phenyl)-4-isoxazolyl penicillins (cloxacillin, dicloxacillin and flucloxacillin) by measuring the absorbance at 338-340 mμ of penicillenic acid formed on heating at 70°C in pH 2.0 buffer solution with copper as a catalyst. The results obtained with samples of cloxacillin, dicloxacillin and flucloxacillin are in good agreement with those obtained by biological assay.

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