A 2'-N-ACETYLATING ENZYME OF AMINOGLYCOSIDES

Abstract

We have found in our stock cultures of clinical origin Providencia strains that were resistant to various aminoglycoside antibiotics including lividomycin and the gentamicin C complex but sensitive to kanamycin A and a new kanamycin A derivative, BB-K8. The substrate profile of inactivation of aminoglycosides combined with structural studies of the inactivated product of 3'-deoxyparomamine, indicate that aminoglycosides were inactivated by acetylation of the amino group at the 2'-C position.