A GLYOXALASE I INHIBITOR OF A NEW STRUCTURAL TYPE PRODUCED BY STREPTOMYCES

Abstract

Many streptomyces strains produced an inhibitor of crude glyoxalase prepared from rat liver which did not inhibit glyoxalase I prepared from yeast. Another inhibitor, C₁₁H₁₄O₆, which inhibited glyoxalases prepared from both rat liver and yeast was obtained from a cultured broth of Streptomyces griseosporeus and crystallized. Preincubation of this inhibitor with reduced glutathione increased its inhibitory activity, which suggested its reaction with reduced glutathione. It showed a strong inhibition of growth of HeLa cells and inhibition of EHRLICH ascites carcinoma by daily injection. It also showed weak inhibition of the solid type of EHRLICH carcinoma and prolonged the survival period of mice inoculated with L-1210 cells.