INHIBITION OF PROTEIN SYNTHESIS IN SACCHAROMYCES CEREVISIAE BY THE 12, 13-EPOXYTRICHOTHECENES TRICHODERMOL, DIACETOXYSCIRPENOL AND VERRUCARIN A

REVERSIBILITY OF THE EFFECTS

Abstract

Inhibition of protein synthesis by trichodermol, diacetoxyscirpenol and verrucarin A in cells and spheroplasts of Saccharomyces cerevisiae was investigated. Inhibition was reversible for trichodermol and diacetoxyscirpenol, both drugs being removed from their target site(s) by washing, but was irreversible for verrucarin A. These results are interpreted in relation to variations in chemical structure between these trichothecenes.