COSYNTHESIS AND PROTOPLAST FUSION BY MUTANTS OF BIALAPHOS (AMPBA) PRODUCING STREPTOMYCES HYGROSCOPICUS

Abstract

Cosynthesis in mixed culture and protoplas fusion of non-producing mutants of Streptomyces hygroscopicuws which may produce biosynthetic intermediates of bialaphos(AMPBA) weres tudied. Non-producing mutants were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine(NTG) treatment, and six stable non-producing mutants were used for cosynthesis and protoplast fusion studies. The biosynthetic block sites of the non-producers were consistent with studies of the biosynthetic pathway of AMPBA.