ACTINOIDIN A₂, A NOVEL GLYCOPEPTIDE: PRODUCTION, PREPARATIVE HPLC SEPARATION AND CHARACTERIZATION

Abstract

An unidentified Nocardia sp. (SK&F-AAJ-193) was isolated and found to produce actinoidin A and a novel analog which we have named actinoidin A₂. This new glycopeptide antibiotic differs from actinoidin A by the presence of rhamnose instead of acosamine. This analog was isolated using Dianion HP-20 resin followed by a specific glycopeptide affinity column (Affigel-10-D-Ala-D-Ala). The purification was accomplished using preparative ion-pairing chromatography. Actinoidin A₂ is active against Staphylococcus aureus and coagulase-negative Staphylococci although it is less potent than actinoidin A.