MYCENON, A NEW METABOLITE FROM A MYCENA SPECIES TA 87202 (BASIDIOMYCETES) AS AN INHIBITOR OF ISOCITRATE LYASE

Abstract

Mycenon (C₁₁H₅Cl₃O₃), a new inhibitor of isocitrate lyase (EC 4.1.3.1) was isolated from the culture broth of a basidiomycete, Mycena sp. Mycenon is a novel chlorinated benzoquinone derivative which is also active against bacteria and fungi. Malate synthase (EC 4.1.3.2) the second key enzyme of the glyoxylate cycle was not affected by mycenon. Isocitrate lyase preparations from plants, bacteria and fungi were sensitive. The following Ki-values for mycenon have been determined: Ricinus communis, 5.2 μM; Acinetobacter calcoaceticus, 11 μM; Neurospora crassa, 7.4 μM. The structure of mycenon has been determined by a single crystal X-ray analysis.