PROBESTIN, A NEW INHIBITOR OF AMINOPEPTIDASE M, PRODUCED BY STREPTOMYCES AZUREUS MH663-2F6 I. TAXONOMY, PRODUCTION, ISOLATION, PHYSICO-CHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES

TAKAAKI AOYAGI; SHIGEMI YOSHIDA; YASUSHI NAKAMURA; YASUHIRO SHIGIHARA; MASA HAMADA; TOMIO TAKEUCHI

doi:10.7164/antibiotics.43.143

The Journal of Antibiotics

Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820

PROBESTIN, A NEW INHIBITOR OF AMINOPEPTIDASE M, PRODUCED BY STREPTOMYCES AZUREUS MH663-2F6

I. TAXONOMY, PRODUCTION, ISOLATION, PHYSICO-CHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES

TAKAAKI AOYAGI, SHIGEMI YOSHIDA, YASUSHI NAKAMURA, YASUHIRO SHIGIHARA, MASA HAMADA, TOMIO TAKEUCHI

Author information

JOURNAL FREE ACCESS

1990 Volume 43 Issue 2 Pages 143-148

DOI https://doi.org/10.7164/antibiotics.43.143

Details

Abstract

Probestin has been isolated as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M from Streptomyces azureus MH663-2F6. It was purified by use of column chromatography of Amberlite XAD-4, silica gel, YMC-gel, Toyopearl HW-40, YMC D-ODS-5 (HPLC) and then isolated as colorless powders. Probestin is competitive with the substrate, and the inhibition constant (Ki) of it was 1.9×10^-8 M.

Content from these authors

Favorites & Alerts

Corresponding author

Register with J-STAGE for free!