AB3217-A, A NOVEL ANTI-MITE SUBSTANCE PRODUCED BY A STRAIN OF Streptomyces platensis

Abstract

AB3217-A, a novel anti-mite substance, was isolated from the fermentation broth of a streptomycete strain. The strain was isolated from a soil collected at Kita-azumi, Nagano Prefecture, Japan, and identified as Streptomyces platensis AB3217.
AB3217-A was purified by Amberlite IR120B, Diaion HP-20 and CM-Sephadex C-25 column chromatographies. The molecular formula was determined as C₁₇H₂₃NO₇ by elemental analysis, MS and ¹³C NMR spectroscopy. The structure of AB3217-A was determined to be (1R, 3S, 4S, -7R, 8R, 11R, 12S, 13R)-4, 12, 13-trihydroxy-8-(4-methoxyphenyl)-6-aza-2, 9, 14-trioxatncyclo-[9.2.1.0^{3, 7}]tetradecane by spectroscopic analysis and X-ray crystallographic analysis. The molecule of AB3217-A has unique structure that deacetylanisomycin and β-D-xylofuranose linked through glycosidic bond and ether bond resulting in the formation of nine-membered ring.
AB3217-A showed marked activity against the two spotted spider mite, Tetranychus urticae.