GLISOPRENINS, NEW INHIBITORS OF ACYL-CoA: CHOLESTEROL ACYLTRANSFERASE PRODUCED BY Gliocladium sp. FO-1513

I. PRODUCTION, ISOLATION AND PHYSICO-CHEMICAL AND BIOLOGICAL PROPERTIES

Abstract

Gliocladium sp. FO-1513 was found to produce novel inhibitors of acyl-CoA: cholesterol acyltransferase (ACAT). Two active compounds, designated glisoprenins A and B, were isolated from the culture broth of the producing strain by a conventional method. The IC₅₀ values of glisoprenins A and B for ACAT activity were 46 and 61 JUM in an enzyme assay using rat liver microsomes, and 1.2 and 0.57μM in a J774 macrophage assay, respectively.