NAGSTATIN, A NEW INHIBITOR OF N-ACETYL-β-D-GLUCOSAMINIDASE, PRODUCED BY Streptomyces amakusaensis MG846-fF3

TAXONOMY, PRODUCTION, ISOLATION, PHYSICO-CHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITIES

Abstract

Nagstatin, a new inhibitor of N-acetyl-β-D-glucosaminidase (NAG-ase) was discovered in the fermentation broth of Streptomyces amakusaensis MG846-fF3. It was purified by chromatography on Dowex 50W, Avicel and Sephadex LH-20 followed by the treatment of active carbon and then isolated as colorless powder. Nagstatin has the molecular formula of C₁₂H₁₇N₃O₆. It is competitive with the substrate, and the inhibition constant (Ki) was 1.7 × 10^-8M.