CYCLOTHIALIDINE, A NOVEL DNA GYRASE INHIBITOR II. ISOLATION, CHARACTERIZATION AND STRUCTURE ELUCIDATION

TSUTOMU KAMIYAMA; NOBUO SHIMMA; TATSUO OHTSUKA; NOBORU NAKAYAMA; YOSHIKO ITEZONO; NAOKI NAKADA; JUNKO WATANABE; KAZUTERU YOKOSE

doi:10.7164/antibiotics.47.37

The Journal of Antibiotics

Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820

CYCLOTHIALIDINE, A NOVEL DNA GYRASE INHIBITOR

II. ISOLATION, CHARACTERIZATION AND STRUCTURE ELUCIDATION

TSUTOMU KAMIYAMA, NOBUO SHIMMA, TATSUO OHTSUKA, NOBORU NAKAYAMA, YOSHIKO ITEZONO, NAOKI NAKADA, JUNKO WATANABE, KAZUTERU YOKOSE

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JOURNAL FREE ACCESS

1994 Volume 47 Issue 1 Pages 37-45

DOI https://doi.org/10.7164/antibiotics.47.37

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Abstract

Cyclothialidine is a novel DNA gyrase inhibitor produced by Streptomyces filipinensis NR 0484. It was isolated from the culture broth by charcoal adsorption, Diaion HP-21, Amberlite CG-50, DEAE Toyopearl, and Toyopearl HW-40 SF column chromatography. The structure of cyclothialidine was determined to be a unique twelve membered lactone by amino acid analysis and various 2D-NMR experiments. Cyclothialidine inhibited Escherichia coli DNA gyrase with an IC₅₀ of 30 ng/ml.

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