The chemical structure of a novel thiopeptide antibiotic, glycothiohexide α (1), isolated from the fermentation broth of a "Sebekia" species was determined based on extensive 2D NMR studies, as well as, IR, UV, and mass spectral data. The chemical structure of glycothiohexide α is closely related to nosiheptide (3) and antibiotic S-54832A.
