GERI-BP002-A, Novel Inhibitor of Acyl-CoA: Cholesterol Acyltransferase Produced by Aspergillus fumigatus F93

Abstract

A new inhibitor of acyl-CoA: cholesterol acyltransferase (ACAT), designated GERI-BP002-A, was isolated from the culture broth of Aspergillus fumigatus F93 by acetone extraction, EtOAc extraction, SiO₂ column chromatography and reverse phase HPLC. Spectroscopic analyses of the compound identified bis (2-hydroxy-3-tert-butyl-5-methylphenyl) methane as the structure and its molecular weight and formula to be 340 and C₂₃H₃₂O₂, respectively. GERI-BP002-A inhibited ACAT activity by 50% at the concentration of 50 μM in an enzyme assay system using rat liver microsomes.