Discovery of Novel Ansamycins Possessing Potent Inhibitory Activity in a Cell-based Oncostatin M Signalling Assay

PAUL STEAD; SHAZIA LATIF; ANDREW P. BLACKABY; PHILIP J. SIDEBOTTOM; ANGELA DEAKIN; NICHOLAS L. TAYLOR; PAUL LIFE; JOHN SPAULL; FIONA BURRELL; ROSIE JONES; JANE LEWIS; IAN DAVIDSON; THOMAS MANDER

doi:10.7164/antibiotics.53.657

PAUL STEAD, SHAZIA LATIF, ANDREW P. BLACKABY, PHILIP J. SIDEBOTTOM, ANGELA DEAKIN, NICHOLAS L. TAYLOR, PAUL LIFE, JOHN SPAULL, FIONA BURRELL, ROSIE JONES, JANE LEWIS, IAN DAVIDSON, THOMAS MANDER

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JOURNAL FREE ACCESS

2000 Volume 53 Issue 7 Pages 657-663

DOI https://doi.org/10.7164/antibiotics.53.657

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Abstract

We describe the isolation and characterisation of novel non-benzoquinone ansamycin metabolites related to geldanamycin from a culture of Streptomyces sp. S6699. The compounds possess potent inhibitory activity in a cell-based assay measuring inhibition of oncostatin M signalling in a reporter cell line utilising a secreted placental alkaline phosphatase (sPAP) readout. In this paper we report the isolation and structure elucidation of the compounds and describe some of their biological properties.

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