1981 Volume 34 Issue 1 Pages 16-20
The antigenicity of fosfomycin (FOM), a new antibiotic agent, was studied.
The binding of 3H-FOM with human serum protein was measured by the short term dialysis method. The binding was very weak only by 2.16% and reversible.
The rabbits were immunized subcutaneously by fosfomycin as sodium salt (FOM-Na) with FREUND'S complete adjuvant. Antigenicity of IgG was examined by means of agar gel precipitation, passive cutaneous anaphylaxis and passive hemagglutination. To study the antigenicity of IgE, the BALB/cA mice were immunized intraperitoneally by FOM-Na with aluminium hydroxide gel and, passive cutaneous anaphylaxis in the rats was used. In these experiments, FOM-Na showed no antibody formation of IgG and IgE.
Consequently, it can be concluded that FOM dose not conjugate with human serum protein to form its hapten and has no antigenicity.
