The Metabolism of Streptomyces Griseus. VII The Oxidation of L-Glutamic Acid by the Intact Mycelium

Abstract

In the preceding paper¹⁾ of this series, it was reported that various amino acids especially glutamic acid were oxidized by the freeze-dried powder of S. griseus grown on the casein medium. But, the freeze-dried powder itself suspended in the phosphate buffer did not oxidized them. The activity of the amino acid oxidation occurred by the incubation for a long time. But, the enzyme solution employed there was a complex system. Recently, the author obtained the mycelium suspension from Dulaney’s synthetic medium supplemented with glutamate, which oxidizes glutamic acid without particular procedures. The extraction of glutamic dehydrogenase from the mass cultured mycelium by grinding was unsuccessful, because the homcgenate contained the excess amount of the mucilage.

In the present study, some results on L-glutamate oxidation by the intact mycelium of S. griseus grown on Dulaney’s medium supplemented with glutamate will be reported.