1961 Volume 14 Issue 4 Pages 221-230
In a previous communication1) of this series, the oxidation of L-glutamic acid by the intact mycelium has been reported. In the present report (Part I), some results on the technical problem for manometric experiments which has not been investigated particularly up to the present, and on the culture condition for the study on L-amino acid oxidase and L-alanine-oxidizing enzyme by the intact mycelium will be reported.
As described in the previous paper2), in the presence of diphosphopyridine nucleotide, DL-alanine was oxidized by the crude enzyme solution obtained from S. griseus, while in the presence of flavin adenine dinucleotide, DL-alanine and L-leucine were not oxidized by it. So, L-leucine and DL-alanine were used as the substrates for L-amino acid-oxidase and alanine oxidizing enzyme.
