Original Articles
Mechanism of Action of Antibiotics. I Mode of Excretion and Accumulation of Aqueous Soluble and Basic Antibiotics in Mouse
1962 Volume 15 Issue 2 Pages 67-72
Details
1962 Volume 15 Issue 2 Pages 67-72
The activities of streptomycin and kanamycin have been measured mainly by a biological assay method such as a cylinder plate or a disc plate method using Bacillus subtilis as the test organism. Since Wilzbach1) developed the unique method of labeling organic compound with tritium (3H) gas, tritiated biochemical material has been a new useful tool in this field.
In 1956, Andre2) showed many beautiful autoradiographs of mouse organs administered with tritiated dihydrostreptomycin and tetracyline to elucidate in whice part of organs such antibiotics were distributed and retained in the body. The present authors labeled dihydrostreptomycin and kanamycin with tritium gas and investigated the fate of these antibiotics in mouse.
Two main new findings were obtained. One is that the radioactivity of tritiated dihydrostreptomycin was detected both in serum and erythricytes. The ither is that a complex of nucleoprotien and dihydrostreptomycin was obtained in vivo.
