Original articles
Selective Toxicity of Blasticidin S to Piricularia Oryzae and Pellicularia Sasakii
1964 Volume 17 Issue 2 Pages 71-74
Details
1964 Volume 17 Issue 2 Pages 71-74
Blasticidin S inhibits the growth of Piricularia oryzae at a concentration below 10 ppm. In a previous report1) it has been shown that 1 ppm of blasticidin S almost completely inhibits the over-all incorporation of 14C-amino acids into microsomal protein in a cell-free system of Piric. oryzae, whereas it inhibits incompletely the amino acid activating enzymes and the transfer of amino acid to sRNA.
On the other hand, a dose of more than 100 ppm of blasticidins is needed to inhibit the growth of Pellicularia sasakii. Thus, Pellic. sasakii is considered to be non-sensitive fungus to blasticidin S2).
Recently, H. Nakamura (1962)3) found that two isolates of Piric. oryzae increased their tolerance for blasticidin S up to 1,000 ppm and 4,000 ppm respectively, by successive transfers on potato dextrose agar containing increasing concentrations of blasticidin S.
To elucidate the nature of the non-sensitiveness in Pellic. sasakii and tolerance in the adapted clone of Piric. oryzae to blasticidin S, the present study has been carried out.
