Abstract
Double immunohistochemistry of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins [synaptosomal-associated protein of 25kDa (SNAP-25), syntaxin and vesicle-associated protein-2 (VAMP-2)], and specific cell markers of taste buds cells [α-gustducin and phospholipase Cβ2 (PLCβ2) for type II cells; neural cell adhesion molecule (NCAM) for type III cells] was applied to gustatory epithelia of the rat circumvallate papillae. All three SNARE proteins were present in some elongated taste buds cells as well as intra-, peri- and subgemmal nerve fibers. Double immunohisotochemistry revealed that nearly all α-gustducin and PLCβ2 immunoreactive cells expressed SNAP-25, syntaxin, and VAMP-2. A majority of NCAM immunoreactive cells showed immunoreactivity for these SNARE proteins. These results indicate that these synapse-associated proteins (SNAP-25, syntaxin and VAMP-2) are present in both type II cells and type III cells. Moreover, more than 50% of intragemmal cells containing SNARE proteins showed immunoreactivities for α-gustducin, PLCβ2, and NCAM, suggesting the possible presence of transitional cells having histochemical proterties of both type II and type III cells.
