A New Type Pycnometer for Biological Specimens with its Application to Isolated Nuclei and Blood Cells

Abstract

In order to prevent some loss of a specimen over-flown out of an ordinary pycnometer, it is nessessary to collect the specimen at the bottom of pycnometer. To solve this restriction, a pycnometer was constructed as a type of tube as possible to centifuge (Fig. 1).
When the volume of specimen is estimated from another measurement such as hematocrit method or micrometry in the unit volume of a specimen suspension, the specific gravity of the specimen is able to calculate from the formula (2). Blood cells and isolated nuclei were used for this example (Table 2 and 3).
The value obtained from the formula (2) indicates an approximate specific gravity of the specimen in the aqueous state. Further, as an extent of the state of specimen, the formula (5) for the dry specimen was induced from the formula (2). The value of the dry-material calculated from the formula (5) indicates an approximate specific gravity of the specimen in the non-aqueous state. The value of isolated nuclei, as an example of the formula (5), was well agreed with that of nuclei reported by ALLFREY et al.