Abstract
The composition and the action of the extract which was extracted from the surface region of the hogs' gastric mucosa by ether-alcohol, followed by a further extraction by alcohol have been studied. The results obtained may be summarized as follows.
1. As a result of paper-chromatographic analysis - using n-Butanol + Acetic acid + Water and saturated 10% NH3 in Butanol as solvent and Ninhydrin reaction-histamine, histidine, tyrosine, methionine and leucine seem to be present in the extract.
2. The subcutaneous injection of the extract into starved rats brings about a remarkable production of secretory granules in the gastric peptic cells. The effects are almost identical with those resulting from the injection of histamine⋅2HCl.
3. The effects of the extract on the contraction of the intestine of guinea pigs (MAGNUS's method) are striking and are very similar to those of 10-6 histamine⋅2HCl solution.
To sum up the results obtained, we may conclude that though the gastric hormone productin (FUJIE) - which is secreted from the gastric surface cells and promotes the production of secretory granules in the peptic cells and contains an imidazol group - seems to be not a simple component but to be composed of some of amino-acids, the action of productin, however, is so very similar to that of histamine as to suggest that it is merely histamine.
