Abstract
For the experiment on the gastric hormone productin (FUJIE) which is believed to be produced in the gastric surface epithelial cells and secreted from them, the gland stomachs of rats were placed in 33% alcohol (for 4, 6, 12 and 24hrs.), and the surface epithelial cells were isolated, so that productin in the cells might be transfused into alcohol.
Histological observation of the stomach after the immersion in alcohol reveals that the separation of the surface epithelial cells is almost complete in the case of 12hr.-immersion.
The extract was centrifuged, the isolated epithelial cells were separated, the supernate was evaporated on the boiling water bath, dissolved in 0.9% NaCl-solution, filtered and then was injected into unfed rats. Materials were taken at the periods of 1/3-2hrs. after the injection, and the gastric chief cells and the exocrine pancreatic cells were observed.
In the 4hr.-extract (the stomach was immersed in alcohol for 4hrs.), a weak factor which promotes the production of secretory granules in the gastric chief cells and the pancreatic cells is found. In 6hr.-extract and 12hr.-extract, the production-factor increases by degrees, but on the other hand, another factor which promotes the vacuolization of the secretory granules in the gastric chief cells, is also increasing, and in 24hr.-extract both factors seem to be abundant.
In 12hr.-extract of the intestine which is extracted using the same methods as in the case of the stomach, no factor is found which promotes the production of secretory granules, and only a weak factor which promotes the discharge of zymogen granules from the pancreatic cells.
Thus, it is concluded that the substance which has an effect similar to that of the gastric hormone productin (promoting factor in the production of secretory granules) on the gastric chief cells and the pancreatic cells is contained in the extract of the gland stomach, and not in the extract of the intestine. Further another factor which promotes the dischage of secretory granules from the gastric chief cells increases with the prolongation of immersion of the stomach. This provides interesting data for further studies in which the component of productin is to be analytically investigated.
