Archivum histologicum japonicum
Print ISSN : 0004-0681
Glycerinated Glycol Methacrylate Mounting Medium in Highlighting Non-Coated or Glycerin-Substituted Microsamples under the Scanning Electron Microscope
Takuro MURAKAMIShozo IRINO
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1976 Volume 39 Issue 3 Pages 157-163

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Abstract
Glycol methacrylate hardened with 10-15% glycerin showed no charging and gave dark contrast in the scanning electron microscope with an acceleration voltage of 25kV and a specimen current of 1×10-10A. Using human blood cells, it Was shown that this conductively treated resin is a useful mounting substrate to highlight the non-coated microsamples or to obtain their highly contrasted images under the scanning electron microscope. It was also demonstrated that the glycerin-substituted blood cells can be scanned with little shrinkage.
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© International Society of Histology and Cytology
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