Abstract
Already, FUJIE and his co-workers reported that secretory function of the pancreatic cell can be divided into two, production and discharge of zymogen granules, and on the other hand, though there are only few investigations, cytoplasmic basophilia (RNA), which greatly related to protein synthesis in the cell, and to the secretory function of pancreatic cells. The author have investigated and compared the functional images of pancreatic cells, especially putting a stress on mitochondria, zymogen granules and RNA.
According to my results obtained in the forgoing research with methyl green-pyronin staining of pancreatic cell, RNA images in pancreatic cells were separated in two parts, the basal part and the granular layer. The former does not show any difference in accordance with the functional movement of the cell, but the latter showed a remarkable difference, for instance, the homogeneous type, the heterogeneous type and the transitional type etc. In the homogeneous type, RNA was lightly stained by pyronin and which spreaded out homogeneously in the granular layer, and the mitochondria showed a vigorous type with new production of zymogen granules. On the contrary, in the heterogeneous type, RNA was distributed like small scabs or granules in the granular layer, and mitochondria did not show any morphological changes, and new production of zymogen granules was ceased. Transitional type consisted of heterogeneous and homogeneous type, and perhaps functionally stood at the mid point between homogeneous and heterogeneous types.
Although, I classified RNA images of the pancreatic cell into 3 types, this is not conclusive. I first investigated RNA images of the pancreatic cell on two points of view, the first is the separation of pancreatic secretory function, a new production and discharge of granules, the other is on the consideration of methyl green-pyronin staining conditions which is very difficult to obtain a constant result. I believe that this investigation will benefit the morhological research of cytoplasmic basophilia (RNA), generally on RNA in secretory cells to some extent.
