Quorum Sensing Control using Synthetic Autoinducer Analogues and Cyclodextrins in Gram-Negative Bacteria

Abstract

To control quorum sensing in gram-negative bacteria, several kinds of autoinducer analogues were synthesized and examined their agonistic or antagonistic activities against quorum sensing in gram-negative bacteria. N-acyl-L-homocystein thiolactones acted as agonists and N-acyl-cyclopentylamides acted as antagonists against autoinducers in gram-negative bacteria. Cyclodextrin-autoinducer complex formation was also investigated. The inhibition effects on autoinducer activities of quorum sensing in gram-negative bacteria was observed by adding α- or β-cyclodextrin in the bacterial culture medium, while γ-cyclodextrin showed no effect.