Detection of SNPs using Oligonucleotide Immobilized Capillary Chromatography

Abstract

In the present work, we investigated the effects of GC content, mismatching position, and length on the thermostability of double-strand oligo-DNAs in order to develop an affinity chromatography using oligonucleotide-immobilized capillary for SNP detection. We found that a single mismatching base pair appeared on 25 mer fragment of human tp53 tumor suppressor protein gene could reduce the melting temperature by 6.6°C. It seemed to be enough for eluting the mutated fragment separately from the intact one during a course of temperature ascending process.