Abstract
INTRODUCTION: Dialyzer apparatus have widely been used as an artificial kidney in medical treatment. However, some side effects as amyloidosis and so on have been occurred during the long term treatment. Therefore, we forcused on development of a hybrid artificial kidney with not only filtration but also reabsorption apparatus, but extensive spreading is easily occurred and difficult to maintain uniform monolayer state on collagen coated substrate. The purpose of this study is improvement of the cell adhesion, uniform stable monolayer formation and active transport function by immobilization of RGD on the culture substratum.MATERIALS AND METHODS: Polycarbonate semipermeable membrane separating culture well was coated by collagen, fibronectin, laminin and synthetic polypeptide including RGD (PnF). Cell adhesive and digoxin transport activities were estimated using renal proximal tubule cell line overexpressed p-glycoprotein gene.RESULTS AND DISCUSSION: Immobilized cell densities at initial and confluent conditions on PnF-coated membrane were higher than those on the other conditions in culture well. Transepithelial electrical resistance and digoxin transport activity on PnF coated membrane were the highest of all conditions. This might be caused by uniform cell morphology and high cell density.
