Asian Pacific Confederation of Chemical Engineering congress program and abstracts
Asian Pacific Confederation of Chemical Engineers congress program and abstracts
Session ID : 1P-01-037
Conference information

Isolation of Alkalophilic Nylon Oligomer Degrading Bacterium, Agromyces sp. KY5R, and Characterization of the Degrading Enzymes
Kengo YasuhiraMasahiro TakeoSeiji Negoro
Author information
CONFERENCE PROCEEDINGS FREE ACCESS

Details
Abstract
Alkalophilic nylon oligomer degrading strains were isolated from wastewater of nylon factory, and from activated sludge of a sewage disposal plant. One isolate, KY5R, showed good growth on LB-NOM10 plate (LB plate containing nylon oligomer mixture, pH10), and produced a clear zone on this plate, while previously isolated Flavobacterium sp. KI72 showed no growth and no halo formation on the LB-NOM10 plate. This result suggests that the insoluble nylon oligomers are degraded at pH10 by the strain KY5R. Nucleotide sequence of the 16S rRNA of the KY5R had 98% homology to that of Agromyces mediolanus, and 2,4-diaminobutyric acid was found as a component of cell wall. From these results, we have concluded that the strain KY5R is classified as Agromyces sp. Enzyme assay using the cell extracts of KY5R suggested that strain KY5R has the exo-type Ahx-oligomer hydrolase (EII) and endo-type Ahx-oligomer hydrolase (EIII) activities, but the no Ahx-cyclic dimer hydrolase (EI) activity. Studies using the antiserum against the purified EII of KI72 showed that the EII enzyme from Agromyces sp. KY5R (A-EII) is immunologically identical to that from Flavobacterium sp. KI72 (F-EII). In addition, DNA hybridization study using the DNA probe for the EIII gene from KI72 (F-nylC) suggested that the EIII enzyme from KY5R (A-EIII) is similar to the enzyme from KI72 (F-EIII).
Content from these authors
© 2004 The Society of Chemical Engineers, Japan
Previous article Next article
feedback
Top