Development and Characterization of EST-SSR Markers for Amur Daylily, Hemerocallis middendorffii Trautv. & C. A. Mey. (Asphodelaceae)

Kazutoshi Masuda; Hiroaki Setoguchi; Koki Nagasawa; Masae Ishihara; Shota Sakaguchi

doi:10.18942/apg.202008

Development and Characterization of EST-SSR Markers for Amur Daylily, Hemerocallis middendorffii Trautv. & C. A. Mey. (Asphodelaceae)

Kazutoshi Masuda, Hiroaki Setoguchi, Koki Nagasawa, Masae Ishihara, Shota Sakaguchi

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Keywords: conservation genetics, EST-SSR, genetic diversity, Hemerocallis, microsatellite, populationgenetics

JOURNAL FREE ACCESS

2021 Volume 72 Issue 1 Pages 73-78

DOI https://doi.org/10.18942/apg.202008

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Abstract

A set of twelve EST-SSR markers were developed to evaluate the genetic diversity and genetic structure of Hemerocallis middendorffii Trautv. & C. A. Mey (Asphodelaceae) in Japan. The total number of alleles for each locus ranged from 3 to 19, with an average of 10.25; the mean values of HO and HE were 0.567 and 0.648, respectively. Versatility of the markers was evaluated for two additional species of the same genus, H. lilioasphodelus L. var. thunbergii (Baker) M. Hotta and H. hakuunensis Nakai to show that all the EST-SSR markers are transferrable between them.

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