Abstract
Based on morphology as well as on karyotype and MIG-seq analyses, two new hybrids, Polygonatum ×hizenense and P. ×sefuriense, from Mt. Sefuri, Pref. Saga, Japan, are described. Polygonatum ×hizenense has 26–29% pollen stainability and 2n = 20, is morphologically intermediate between P. falcatum (with 78–98% pollen stainability and 2n = 18) and P. macranthum (with 95–100% pollen stainability and 2n = 22), which occur parapatrically on Mt. Sefuri. A Neighbor Net network based on MIG-seq data showed P. ×hizenense to be positioned between the P. falcatum cluster and the P. inflatum-P. macranthum cluster, also indicating it to be of hybrid origin between P. falcatum and P. macranthum. Likewise, P. ×sefuriense has 29–37% pollen stainability and 2n = 19, morphology intermediate between P. amabile (with 74–92% pollen stainability and 2n = 20) and P. falcatum, which also occur parapatrically on Mt. Sefuri. The MIG-seq analysis showed P. ×sefuriense to be positioned between the P. falcatum cluster and the P. amabile-P. lasianthum var. lasianthum cluster in the obtained network, indicating it to be of hybrid between P. amabile and P. falcatum. In addition, our analysis based on MIG-seq suggested that P. lasianthum var. coreanum should be separated from var. lasianthum as a distinct species, P. taquetii.
