Biological Indicators of Lifestyle-related Diseases and Urinary 8-Hydroxydeoxyguanosine Level

Abstract

We investigated the relationship between urinary 8-hydroxydeoxyguanosine (8-OH-dG), a marker of oxidative DNA damage, and various lifestyle-related diseases. The subjects were 361 local government employees in Japan. We used a self-reported questionnaire and analyzed data from 280 subjects, concerning gender, body mass index, drinking and cigarette smoking status, and exercise frequency along with the data of physical examinations such as biochemical blood and urine tests accompanied by medical check-up results. Urine specimens were collected to measure 8-OH-dG and creatinine levels. The creatinine-adjusted urinary 8-OH-dG level was used as the indicator of 8-OH-dG excretion. The urinary 8-OH-dG levels were significantly higher in cigarette smokers than in non-smokers, and were significantly higher in subjects in their 50’s than those in their 30’s. No significant relationships were observed between urinary 8-OH-dG level and each lifestyle-related disease, such as obesity, hypertension, dyslipidemia, impaired glucose tolerance, impaired liver function, and hyperuricemia. This study suggested that the urinary 8-OH-dG level was increased in high-age group or by the cigarette smoking, but these lifestyle-related diseases might not affect urinary 8-OH-dG excretion.