Abstract
The tissue culuture of Alaria cordata was carried out to get clone plants for line test and to secure breeding materials. When using the leaf segment of Alaria cordata, MS agar medium contained 2,4-D 0.5mg/1. was most suitable to callus formation. There were no conspicuous differences in the term of culture. The embryoid occured when the formed callus was moved to 1/2MS medium(hormone free), and the young clone plants were regenerated. However, many variants were seen in embryoids stage, therefore the normal individual must be selected while culturing. In 13 strains collected at Okayama prefecture, the regenerative percentages of young clone plants varied in strains greatly. Acclimation of regenerated young plants under controlled humidity was very difficult.
