Applied Forest Science
Online ISSN : 2189-8294
Print ISSN : 1342-9493
ISSN-L : 1342-9493
Tissue culture of cherry tree leaf segments and the effect of auxin : Callus and adventitious root formation
Naoya Fujiwara
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1999 Volume 8 Pages 151-154

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Abstract
Crossbreeding and tissue culture of cherry tree were studied. Adventitious buds of a protected variety of cherry tree called Sikizakura'from Katuyama City were cultured for 60 days. After a small callus formed on the leaves and stems, leaf segments were suspension cultured in lmL MS liquid medium to try to regenerate the plants. The medium was augmented with one of the following combinations: NAA and IBA, IAA and IBA, IAA and BA, 2,4-D and BA, or 2,4-D and IBA. The cultures were maintained in the dark at 24℃. Very little callus formed under a wide range of hormone conditions. Adventitious root developed from callus with a high concentration of NAA, but the leaf segments withered with the other combinations.The calluses were also suspension cultured in MS liquid medium with different light conditions, and concentrations of medium and sugar for 60 days, without effect. A different species of cherry tree, Gyoiko・(Prunus lannesiana cv. ascendens) from Tessei City, was examined. Leaf segments were cultured on MS agar medium with 100uM NAA. In this case, a large callus formed on the leaf cross-section and adventitious roots developed after 7 days. A small callus was formed when a third cherry, Daigozakura・(Prunus pendula form, ascendens) from Ochiai City, was cultured, but it withered. No adventitious buds developed in any case.
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