2015 Volume 63 Issue 1 Pages 1-8
We investigated a way of isolating axenic protoplast of Pyropia yezoensis and the effect of twelve antibiotics on the survival and growth of the protoplasts. The axenic protoplasts were able to be isolated, by carrying out a series of treatments with 0.5% sodium citrate seawater. The survival of the protoplasts was not almost influenced by neomycin (FRM), ampicillin (ABPC), penicillin G (PCG) and streptomycin (SM) during a culture period of 2 weeks. The growth rates of the protoplasts treated with FRM and ABPC were 1.1-2.7 times higher compared with the control, while PCG and SM suppressed growth. More than 80% of the protoplasts treated with FRM formed rhizoidal cells. Treatment with FRM, ABPC, PCG and SM is thought to be a practical method for culture of axenic protoplasts. Our data would contribute to the clarification of the factors involved in the survival, growth and morphogenesis of the protoplasts of P. yezoensis.
