Genetic Variability of DNA Fingerprinting in 5 strains of Red Sea Bream, Pagrus major
1995 Volume 43 Issue 4 Pages 491-497
Details
1995 Volume 43 Issue 4 Pages 491-497
Genetic variability of five strains of red sea bream was investigated by DNA fingerprinting (DNA-FP) using samples of one non-selected strain (S1), 3 strains of growth rate-selected fish (S2 S3, S4), one strain of hybridized and growth-rate selected fish (S5), and wild population. Extracted DNA of these strains were digested by Hinf I restriction enzyme and hybridized with 33.15 and YNZ 22 probes. Band sharing indices (BSI) were calculated from the banding pattern of DNA-FP.
Mean BSI of DNA-FP detected with the 33.15 multilocus probe were relatively high in S2 (0.382) and S3 (0.301), and relatively low in S1 (0.226), S4 (0.166) and S5 (0.206) comparing with the very low value of the wild population (0.120) . There were no differences in BSI values obtained by the two probes.
These results were agreed well with allozyme analysis, and it is concluded that DNA-FP can be used as a marker for monitoring change in genetic variability in artificially propagated fishes for aquaculture.
